EN17424:2020isaEuropeanstandardthatspecifiesamethodforthedeterminationofaflatoxinsinspicesotherthanCapsicumspp.(i.e.,spicesexcludingchilipeppers)usingimmunoaffinitycolumn(IAC)clean-upfollowedbyhigh-performanceliquidchromatography(HPLC)withpost-columnderivatizationandfluorescencedetection(FLD).ThestandardprovidesavalidatedanalyticalproceduretoquantifyaflatoxinsB1,B2,G1,andG2invariousspices,ensuringaccuracy,reproducibility,andcompliancewithfoodsafetyregulations.Itincludesdetailedstepsforsamplepreparation,extraction,purificationthroughIAC,chromatographicseparation,derivatization(e.g.,usingbromineorphotochemicalmethods),andquantificationviafluorescencedetection.Thismethodisdesignedtomeetregulatoryrequirementsformonitoringaflatoxincontaminationinspices,helpingtoprotectconsumerhealthandfacilitatetradebyprovidingareliableandstandardizedtestingapproach.