Monogalactosyldiacylglycerol(MGDG)deficiencyintobaccodisruptsthecytochromeb(sub)6(sub)f-mediatedintersystemelectrontransport.ThisstudyinvestigatestheimpactofMGDGdepletiononthephotosyntheticelectrontransportchain,specificallyfocusingonthecytochromeb(sub)6(sub)fcomplex.ThefindingsrevealthatMGDGplaysacriticalroleinmaintainingtheefficiencyofintersystemelectrontransfer,highlightingitsimportanceinphotosynthesis.TheresearchprovidesinsightsintothestructuralandfunctionalrelationshipbetweenMGDGandthecytochromeb(sub)6(sub)fcomplex,contributingtoabetterunderstandingoflipid-proteininteractionsinphotosyntheticmembranes.