LimmaisapowerfulstatisticalmethodwidelyusedfordifferentialexpressionanalysesinbothRNA-sequencingandmicroarraystudies.Itemployslinearmodelstoassessgeneexpressionchangesacrossdifferentexperimentalconditions,offeringrobustandflexibleanalysiscapabilities.Limmaisparticularlyeffectiveforhandlingsmallsamplesizesandcomplexexperimentaldesigns,makingitapopularchoiceingenomicsresearch.ItsabilitytoincorporateempiricalBayesmoderationimprovesvarianceestimation,enhancingthereliabilityofresults.ThemethodisimplementedintheRprogrammingenvironment,providingresearcherswithacomprehensivetoolsetforhigh-throughputdataanalysis.
