Thestudytitled"CloningofaPotatoProteinaseInhibitorGenePINII-2xfromDiploidPotato(SolanumphurejiaL.)andTransgenicInvestigationof"focusesonthemolecularcloningandfunctionalanalysisoftheproteinaseinhibitorgenePINII-2xfromadiploidpotatospecies,SolanumphurejiaL.Proteinaseinhibitorsplayacrucialroleinplantdefensemechanismsagainstpestsandpathogensbyinhibitingproteolyticenzymes.ThisresearchinvolvestheisolationandcharacterizationofthePINII-2xgene,followedbyitsintroductionintotransgenicplantstostudyitsexpressionandpotentialeffectsonenhancingresistancetobioticstresses.Thefindingscouldcontributetothedevelopmentofgeneticallymodifiedcropswithimprovedpestresistance,offeringinsightsintothemolecularbasisofplantdefensesystems.Thisworkholdssignificanceforagriculturalbiotechnologyandcropimprovementstrategies.