SytoxGreennucleicacidstainisahigh-affinityfluorescentdyeusedtolabelnucleicacidsindeadcells.Itisacell-impermeantdye,meaningitonlyenterscellswithcompromisedmembranes,makingitavaluabletoolfordistinguishingbetweenliveanddeadcellsinviabilityassays.Onceinsidethecell,SytoxGreenbindstoDNAandRNA,exhibitingasignificantincreaseinfluorescenceuponbinding.Thedyeisexcitedbybluelight(around504nm)andemitsgreenfluorescence(approximately523nm).Itiscommonlyusedinflowcytometry,microscopy,andothercell-basedassaystoassesscellviability,apoptosis,andnecrosis.SytoxGreenisknownforitshighsensitivity,lowbackgroundsignal,andcompatibilitywithotherfluorescentprobes,makingitapopularchoiceforresearchersstudyingcelldeathmechanisms.
